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REPTILIAN Genetic Services

DNA SEXING FOR REPTILES

Reptiles have a different mechanism to determine sex, Although the specific molecular mechanism that determines sex has not been revealed in any reptilian species, general modes of sex determination can be described [Bull, 1980; Janzen and Paukstis, 1991]. An individual’s genotype at one or more loci can control whether it develops testes or ovaries. Species that display this mechanism are said to have genotypic sex determination, or GSD. Species with GSD may or may not have distinct sex chromosomes (ZZ males and ZW females or XY males and XX females). A frequent alternative to GSD is environmentally triggered polyphenism (i.e., a single individual can develop testes or ovaries depending upon environmental conditions). Such species are said to have environmental sex determination, or ESD. Various environmental factors, including photoperiod, social environment, and temperature, influence sex determination across the animal kingdom [Bull, 1983; Korpelainen, 1990]. However, temperature is the only environmental variable that has been conclusively shown to determine sex in reptiles [Bull, 1980; Janzen and Paukstis, 1991; Valenzuela, 2004]. This form of ESD is called temperature dependent sex determination, or TSD.

Beaks performs sex identification of reptiles using sex determining gene expression. We are working for following reptiles

Green Lizard

Iguana

Snakes

Breaded Dragon

Turtle

To do this test we use single mitochondrial genes for species identification.

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